| 质粒名称: | pFRT/TO/GFP-SCAF4 (CRISPR_resistant) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 122471 |
| 存储实验室: | Jesper Svejstrup |
|
载体骨架基本信息 |
|
| 载体名称: | pFRT/TO/GFP |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6032 bp |
| 完整质粒大小: | 9392 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SCAF4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | Synonymous mutations conferring resistance to gRNAs described in Gregersen et al. |
| GenBank编号 | NM_020706.2 |
| 启动子 | CMV |
| 标签/融合蛋白: | GFP（主干上的N终端） |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV forward |
| 3' 测序引物 | BGH Reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pFRT/TO/GFP-SCAF4 (CRISPR_resistant) was a gift from Jesper Svejstrup (Addgene plasmid # 122471 ; http://n2t.net/addgene:122471 ; RRID:Addgene_122471) |
| 参考文献部分: | SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, Stewart A, Svejstrup JQ. Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 10.1016/j.cell.2019.04.038. 10.1016/j.cell.2019.04.038 PubMed 31104839 |
买家导航
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)载体质粒基本信息
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)质粒图谱载体图谱和pFRT/TO/GFP-SCAF4 (CRISPR_resistant)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)质粒载体简介
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)质粒序列载体序列
LOCUS pFRT/TO/GFP-SCAF4 (CRISPR_resistant) 9392 bp ds-DNA circular SYN 10-APR-2019
DEFINITION Dox-inducible GFP (N-terminal tag) SCAF4 expression.
ACCESSION .
VERSION .
KEYWORDS pFRT/TO/GFP-SCAF4 (CRISPR_resistant)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9392)
TITLE SCAF4 and SCAF8, mRNA Anti-Terminator Proteins
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9392)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 10, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9392
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 164..185
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind 234..258
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(3709..3733)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 3786..3827
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
primer_bind complement(3865..3882)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3871..4095
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(4228..4247)
/label=F1ori-R
/note="F1 origin, reverse primer"
protein_bind 4379..4426
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 4434..5456
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
"
polyA_signal 5586..5707
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5623..5642)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5677..5696
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5756..5772)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5756..5772)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5769..5791)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5780..5796
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5804..5834)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5849..5870
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5987..6004)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6158..6746)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6238..6257)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6917..7777)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7540..7559
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7778..7882)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7957..7976)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8148..8527
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 8528..8731
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 8682..8702
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 8733..8751
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 8754..8772
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 8782..8806
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 8903..227
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(8948..8969)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(9209..9228)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
ORIGIN
1 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
61 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
121 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
181 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagaag cttacaagtt
241 tgtacaaaaa agcaggcttc accatggacg ccgtcaacgc cttcaaccag gagctctttt
301 cgcttatgga tatgaaacct cccatctcta gagccaagat gattctcatc actaaagctg
361 ctattaaagc tattaagctt tataagcatg tagttcaaat agtagaaaag ttcatcaaaa
421 agtgtaaacc agaatacaag gttccgggat tatatgtaat tgactcaatt gtgcgacagt
481 ctcgtcatca gtttggaact gataaagatg tttttgggcc aagattctct aaaaacataa
541 ctgccacatt ccaatattta tatctttgtc catctgaaga taagagtaaa atagttcgtg
601 tgctgaacct ttggcaaaaa aatggagtgt tcaaaattga aattattcaa cctcttttgg
661 acatggcagc gggaaccagt aatgcagccc cagtagcaga aaatgttacc aataatgaag
721 gctcacctcc acctccagta aaagtttctt ctgaacctcc cacacaagcc actccaaact
781 ccgtgcctgc agttcctcaa ttacccagct ctgatgcttt tgctgctgtg gctcaactct
841 tccaaactac acagggccaa cagcttcagc agatccttca gacttttcaa cagcctccaa
901 aaccacagtc tcctgccctt gacaatgctg tgatggctca ggttcaggct atcacagctc
961 agttaaagac aactcctaca caaccatctg aacaaaaagc tgctttcccc ccacctgaac
1021 agaaaactgc atttgacaag aagttgcttg atagatttga ctatgatgat gagccagaag
1081 ctgtggaaga atcaaagaaa gaggatacca ctgccgtcac cacgacagca cctgctgccg
1141 cagtaccccc tgcacccacc gccaccgtgc ctgctgctgc tgcacccgct gctgcctctc
1201 ctcctcctcc acaggcacca tttggctttc ctggagatgg catgcagcag cctgcttata
1261 cgcaacacca gaatatggat caattccaac ctcgtatgat gggaatacaa caggatccaa
1321 tgcaccatca ggttccactt cctcctaatg gacaaatgcc aggatttgga cttcttccta
1381 cacctccatt tcctcccatg gctcagcctg tgattcctcc aactccacca gtgcagcagc
1441 ctttccaagc ttcttttcag gcacaaaatg aaccacttac acagaagccg catcagcagg
1501 aaatggaagt agaacaacct tgtattcaag aggttaagcg acatatgtct gataacagaa
1561 agtcaagatc taggtcagca tccaggtcac caaaaaggag gcgatctaga tctggttcta
1621 gatctcgaag gtctcggcat cgacgttctc gatctcggtc cagggataga cgccgacatt
1681 ctccccgatc tcgatctcaa gaaagacggg atcgagaaaa agagagagaa cgtcgacaaa
1741 aaggcctccc tcaagtgaaa ccggaaactg caagtgtttg cagtactacc ctctgggtgg
1801 ggcagctgga caaaagaact actcagcagg atgttgccag tctcttggaa gagtttggtc
1861 caattgaatc aattaatatg attcctccca ggggttgtgc ctatattgtt atggttcata
1921 ggcaagatgc ctatcgtgcc ctgcagaaac tgagccgagg aaactataaa gtgaaccaga
1981 aatccataaa gattgcctgg gccttaaaca aaggaataaa ggcagattat aagcagtatt
2041 gggatgtaga acttggtgtt acttatattc catgggacaa agtcaagcct gaggaactgg
2101 agagtttttg tgaaggagga atgttggaca gtgacacact taacccagat tggaaaggaa
2161 ttcctaagaa gcctgaaaat gaagttgctc aaaatggagg tgctgaaacc tcacacacag
2221 aaccagtatc acccatacct aaaccattac ctgtgcctgt ccctcctatt cctgttcctg
2281 cacctataac agtgccacct ccacaggtcc caccacatca accgggtcca cctgtagttg
2341 gtgctctcca gccgcctgct ttcacgcctc ctctgggaat accgcctcca ggctttggtc
2401 ctggtgttcc tcctccccct cctcctccac catttttgcg cccaggattc aacccaatgc
2461 atttaccacc aggttttctg cctcctggac ccccacctcc tataactcca ccagtatcca
2521 ttcctcctcc tcacactcca ccaataagca tcccaaactc tactatcgct ggtataaatg
2581 aagacactac aaaagactta tctattggaa atcccattcc aacagtggtg tctggggcta
2641 gaggaaacgc cgagtctggt gacagcgtga aaatgtatgg ctctgccgtg ccacctgctg
2701 cacccacgaa tctgcccacc cctcctgtaa cccagcctgt ttcacttctt ggcactcaag
2761 gagttgcccc tggtcctgta attggacttc aggcaccatc tactggtctt cttggcgccc
2821 ggcccggtct catcccactc cagcgccctc caggaatgcc cccacctcac ttacagcggt
2881 tccctttgat gccgccccgt cccatgccac cgcacatgat gcacagaggc ccaccgccag
2941 gaccaggggg ctttgcgatg cctccacctc atggaatgaa aggtcccttc ccaccgcatg
3001 gcccctttgt taggcctggt ggaatgccag ggctcggggg cccagggcca ggcccagggg
3061 gtcctgaaga cagagacgga aggcaacagc cgccgcagca gccacagcag cagccacagc
3121 cgcaggcgcc ccagcaacca cagcagcagc agcagcagca gccaccacca tcacaacagc
3181 ctccaccaac acagcagcag ccacagcagt ttagaaatga taacaggcag cagttcaatt
3241 caggtagaga ccaagaaagg tttggaagaa gatcttttgg aaatagggtg gaaaatgacc
3301 gggaacggta tgggaaccgt aatgatgata gagataatag taaccgtgac aggagagagt
3361 ggggaaggag gagccctgac cgggacaggc acagagactt ggaagagaga aatagacgct
3421 ctagtgggca tcgagacaga gagagagatt ctagagatag agagtctcgt agagagaagg
3481 aagaagcccg aggaaaggaa aagcctgagg tgacagacag ggcaggtggt aacaaaaccg
3541 ttgaacctcc cattagccaa gtgggaaatg tagacactgc ttcagaactt gagaaggggg
3601 tgtctgaggc tgcagtccta aagccttctg aagagttacc tgctgaggct acctcatccg
3661 ttgaacccga aaaggattct ggctcagcag cagaggctcc tcgttaaaac ccagctttct
3721 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc aagtctagag ggcccgcggt
3781 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgctc gagtctagag
3841 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
3901 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
3961 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
4021 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
4081 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
4141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
4201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
4261 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
4321 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtacctaga
4381 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg gccaaaaagc
4441 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
4501 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
4561 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
4621 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
4681 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
4741 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
4801 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
4861 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
4921 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
4981 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
5041 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
5101 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
5161 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
5221 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
5281 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
5341 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
5401 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg
5461 tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
5521 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
5581 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
5641 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
5701 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
5761 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
5821 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
5881 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
5941 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
6001 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
6061 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
6121 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
6181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
6241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
6301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
6361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
6421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
6481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
6541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
6601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
6661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
6721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
6781 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
6841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
6901 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
6961 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
7021 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
7081 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
7141 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
7201 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
7261 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
7321 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
7381 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
7441 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
7501 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
7561 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
7621 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
7681 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
7741 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
7801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
7861 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
7921 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
7981 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
8041 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
8101 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
8161 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
8221 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
8281 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
8341 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
8401 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
8461 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
8521 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
8581 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
8641 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
8701 tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag atctccctat
8761 cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
8821 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactctagc
8881 gtttaaactt aagcttggta ccatggtgag caagggcgag gagctgttca ccggggtggt
8941 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
9001 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
9061 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
9121 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
9181 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
9241 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
9301 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
9361 catggccgac aagcagaaga acggcatcaa gg
//
